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Updated: Jun 24, 2025

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Published on: June 17, 2012
Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high
Lu Zhang1, Lu Wang1, Yongchen Fang1
1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China.
High light stress triggers anthocyanin accumulation in pears via the PuHB40 transcription factor. This process is regulated by reactive oxygen species (ROS) and protein phosphatase 2A (PP2A), enhancing plant stress tolerance.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Plants face increasing environmental stresses due to climate change.
- Stress leads to reactive oxygen species (ROS) accumulation, causing cell damage.
- Anthocyanins are key antioxidants, but ROS-induced accumulation mechanisms are unclear.
Purpose of the Study:
- To investigate the mechanism of ROS-induced anthocyanin accumulation in pears under high-light stress.
- To identify key regulatory factors involved in this pathway.
Main Methods:
- Investigated the role of HD-Zip I transcription factor PuHB40.
- Analyzed the interaction between PuHB40, PuMYB123-like, and PubHLH3.
- Studied the regulation by protein phosphatase PP2A and ROS levels.
Main Results:
- PuHB40 mediates ROS-dependent anthocyanin biosynthesis under high-light stress.
- PuHB40 induces the PuMYB123-like-PubHLH3 complex for anthocyanin production.
- ROS maintains PuHB40 phosphorylation, enhancing transcription and anthocyanin biosynthesis.
Conclusions:
- A novel pathway for ROS-induced anthocyanin biosynthesis in pears is revealed.
- The findings clarify abiotic stress responses and offer insights for improving plant stress tolerance.
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