Related Experiment Video
Updated: Jun 24, 2025
08:37
Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells
Published on: March 10, 2023
2.5K
Protocol for observing lipid droplet dynamics in chicken cone cells
Huimin Pan1, Jinglin Zhu1, Xun Huang2
1State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
STAR Protocols
|June 6, 2024
Summary
This study details a protocol for analyzing lipid droplet dynamics in chicken cone cells using both in vivo and ex vivo retinal cultures. The method involves retinal dissection, electroporation, culture, and lipid droplet staining for organelle research.
Area of Science:
- Cell Biology
- Retinal Research
- Lipid Metabolism
Background:
- Chicken cone cells are valuable models for studying lipid droplet regulation.
- Understanding lipid droplet dynamics is crucial for various cellular processes.
Purpose of the Study:
- To present a detailed protocol for investigating lipid droplet dynamics in chicken cone cells.
- To enable research on lipid droplets using both in vivo and ex vivo retinal models.
Main Methods:
- Dissection of chicken embryo retinas.
- Electroporation and ex vivo/in vitro culture of retinal tissues.
- Staining of lipid droplets using neutral lipid dye.
Main Results:
- A comprehensive protocol for studying cone cell lipid droplets is established.
- The protocol is adaptable for investigating other retinal organelles.
Conclusions:
- This protocol provides a robust method for studying lipid droplet dynamics in chicken cone cells.
- The methodology can be extended to explore diverse organelle functions within retinal tissues.
