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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Related Experiment Video

Updated: Jun 24, 2025

CRISPR-Mediated Reorganization of Chromatin Loop Structure

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

Looping forward: exploring R-loop processing and therapeutic potential.

Kalliopi Stratigi¹, Athanasios Siametis^1,2, George A Garinis^1,2

¹Institute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece.

|June 6, 2024

Summary

This summary is machine-generated.

R-loops, structures formed by RNA invading DNA, are crucial for gene regulation but can cause genome instability if persistent. Understanding R-loop processing offers therapeutic potential for related diseases.

Keywords:

DNA damage DNA repair R‐loops genome instability transcription

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Last Updated: Jun 24, 2025

CRISPR-Mediated Reorganization of Chromatin Loop Structure

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Published on: September 14, 2018

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

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Area of Science:

Molecular Biology
Genetics
Genomics

Background:

R-loops are three-stranded nucleic acid structures formed during transcription.
These structures play roles in gene expression, DNA repair, and cellular homeostasis.
Unresolved R-loops are linked to genome instability and associated diseases.

Purpose of the Study:

To provide a comprehensive overview of R-loop processing.
To explore the dual role of R-loops in cellular functions.
To discuss therapeutic strategies targeting R-loop accumulation.

Main Methods:

Literature review of R-loop formation, processing, and resolution.
Analysis of the physiological and pathological implications of R-loops.
Exploration of therapeutic interventions targeting R-loop dynamics.

Main Results:

R-loop processing can yield beneficial outcomes in gene regulation.
Persistent R-loops contribute to genome instability and disease.
Factors involved in R-loop resolution are critical for maintaining genomic integrity.

Conclusions:

R-loop processing is a critical determinant of cellular outcomes.
Targeting R-loop accumulation presents a promising therapeutic avenue.
Further research into R-loop biology is essential for developing novel treatments.