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Related Concept Videos

CRISPR01:59

CRISPR

50.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jun 24, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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AutoESDCas: A Web-Based Tool for the Whole-Workflow Editing Sequence Design for Microbial Genome Editing Based on the

Chunhe Yang1,2,3, Yi Yang2,3, Guangyun Chu3,4,5

  • 1College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

ACS Synthetic Biology
|June 7, 2024
PubMed
Summary

AutoESDCas is a new tool for designing microbial genome editing sequences. It supports the entire workflow, improving efficiency and reducing off-target edits for high-throughput strain modification.

Keywords:
CRISPR/Cas-mediated homologous recombinationediting sequence designgenome editinghigh-throughput strain modificationoff-target analysiswhole-workflow design

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bioinformatics

Background:

  • Genome editing is crucial for modifying engineered microbes.
  • Designing editing sequences (primers, sgRNA) is vital for accurate and efficient genome editing.
  • Existing tools for whole-workflow editing sequence design lag behind high-throughput experimental needs.

Purpose of the Study:

  • To develop an end-to-end online tool for designing editing sequences in microbial genome editing.
  • To provide a comprehensive solution for diverse genetic manipulation requirements and design scenarios.
  • To empower high-throughput microbial strain modification.

Main Methods:

  • Development of AutoESDCas, an online tool for microbial genome editing sequence design.
  • Integration of CRISPR/Cas system for genome editing.
  • Inclusion of an off-target risk assessment function for editing sequences.

Main Results:

  • AutoESDCas facilitates all types of genetic manipulation for microbial genome editing.
  • The tool enables rapid and efficient acquisition of all necessary editing sequences for the entire genome editing process.
  • Off-target risk assessment significantly improves the usability of design results.

Conclusions:

  • AutoESDCas addresses the need for whole-workflow design tools in microbial genome editing.
  • The tool enhances efficiency and accuracy in high-throughput strain modification.
  • AutoESDCas is freely available, promoting broader accessibility for researchers.