[Recent progress of chromatographic techniques for antibody purification]

Area of Science:

• Biotechnology
• Pharmaceutical Sciences
• Chemical Engineering

Background:

• Antibody drugs are vital for disease diagnosis and therapy, with increasing demand driving production scale.
• Downstream antibody purification is a significant bottleneck, contributing 50-80% to total production costs.
• Chromatographic technology is the primary method for antibody purification due to its selectivity and efficiency.

Purpose of the Study:

• To review recent advancements in chromatographic techniques for antibody purification.
• To discuss the challenges and future developments in antibody purification technology.
• To highlight methods for improving yield, quality, and cost-effectiveness.

Main Methods:

• Affinity chromatography (e.g., Protein A) for antibody capture.
• Ion-exchange, hydrophobic interaction, and mixed-mode chromatography for intermediate purification and polishing.
• Emerging techniques like affinity biomimetic chromatography, hydrophobic charge-induction chromatography, and temperature-responsive chromatography.

Main Results:

• Protein A chromatography is standard but costly.
• Alternative methods like affinity biomimetic and hydrophobic charge-induction chromatography offer comparable purity at lower cost.
• Temperature-responsive chromatography enables mild elution, preventing antibody aggregation and inactivation.

Conclusions:

• Combining different chromatographic methods enhances separation selectivity and mild elution conditions.
• Optimizing chromatographic strategies is crucial for improving antibody yield and quality.
• Future developments focus on cost-effective, efficient, and mild antibody purification techniques.