CircRNA hsa_circ_0069,399 as a potential clinical prognostic marker in laryngeal squamous cell carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Circular RNAs (circRNAs) play a role in cancer. This study identified hsa_circ_0069,399 as a potential prognostic biomarker for laryngeal squamous cell carcinoma (LSCC) due to its upregulation in tumor tissues.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Circular RNAs (circRNAs) are increasingly recognized for their roles in tumor progression, including invasion, metastasis, and apoptosis.
- The specific involvement of circRNAs in laryngeal squamous cell carcinoma (LSCC) pathogenesis remains largely uncharacterized.
- Identifying novel biomarkers is crucial for improving LSCC diagnosis and prognosis.
Purpose Of The Study
- To investigate circRNA expression profiles in LSCC tissues compared to adjacent non-tumorous tissues.
- To identify potential circRNA biomarkers for LSCC.
- To explore the regulatory network of circRNAs in LSCC.
Main Methods
- Transcriptomic sequencing and bioinformatics analysis were employed to profile circRNAs in LSCC and adjacent tissues from ten patients.
- A competitive endogenous RNA (ceRNA) network was constructed to elucidate circRNA-miRNA-mRNA interactions.
- Quantitative real-time PCR (RT-qPCR) and immunohistochemistry were used for validation of key circRNAs and mRNAs.
Main Results
- A total of 126 differentially expressed circRNAs were identified, with 40 upregulated and 86 downregulated.
- Ninety-two circRNA-miRNA-mRNA regulatory axes were linked to clinical immunohistochemical indicators, focusing on five candidate circRNAs.
- The circRNA hsa_circ_0069,399 was found to be significantly upregulated in LSCC tissues and involved in regulatory axes with MKI67 and TP53.
Conclusions
- The circRNA hsa_circ_0069,399 exhibits differential expression in LSCC.
- hsa_circ_0069,399 demonstrates potential as a diagnostic or prognostic biomarker for laryngeal squamous cell carcinoma.
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