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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Pathologists light level preferences using the microscope-study to guide digital pathology display use.

Charlotte Jennings1,2, Darren Treanor1,2,3, David Brettle1

  • 1National Pathology Imaging Co-operative, Leeds Teaching Hospitals NHS Trust, Leeds, UK.

Journal of Pathology Informatics
|June 7, 2024
PubMed
Summary
This summary is machine-generated.

Pathologists rarely adjust digital display brightness, despite its importance. Microscope brightness preferences do not predict display needs, but most pathologists prefer displays around 300 cd/m², with 500 cd/m² suitable for all.

Keywords:
Ambient lightingDigital pathologyDisplay luminanceDisplay recommendationsMicroscope brightness

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Area of Science:

  • Digital Pathology
  • Medical Imaging
  • Human-Computer Interaction

Background:

  • Digital pathology lacks guidelines for display selection and configuration.
  • Pathologists' microscope brightness preferences were hypothesized as predictors for digital display settings.

Purpose of the Study:

  • To investigate pathologists' brightness adjustment habits on microscopes and digital displays.
  • To determine optimal digital display luminance preferences for pathologists.
  • To assess the correlation between microscope and display brightness preferences.

Main Methods:

  • An online survey of 108 pathologists across six NHS hospitals.
  • A practical assessment with 20 consultants to determine display luminance preferences.
  • Development of a novel lightmeter adaptation for measuring microscope eyepiece light output.

Main Results:

  • 81% of pathologists adjust microscope brightness, versus 11% for digital displays.
  • Display adjustments were primarily for visual comfort and ambient light, not tissue factors.
  • Microscope brightness preferences did not correlate with display preferences, except at very high microscope brightness.
  • Most pathologists preferred display luminance <500 cd/m², with 90% preferring 350 cd/m² or less.

Conclusions:

  • Microscope brightness preferences are poor predictors of display luminance needs.
  • A display brightness of 500 cd/m² is suitable for nearly all pathologists; 300 cd/m² suffices for most.
  • The ability to adjust display luminance is important to pathologists, though infrequently used.