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Updated: Jun 24, 2025
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Optimization of a modified Captiva EMR-lipid method based on micro-matrix solid-phase dispersion coupled with gas
N Carro1, R Fernández1, J Cobas1
1Instituto Tecnolóxico para o Control Do Medio Mariño de Galicia, INTECMAR, Consellería Do Mar, Xunta de Galicia, Peirao de Vilaxoán s/n, 36611 Vilagarcía de Arousa, Spain. ncarro@intecmar.gal.
Abstract:
This work describes a reliable, cheap, easy and fast method for analysis of nine bisphenols in mussel samples by gas chromatography-mass spectrometry after trimethylsilylation. The modified method consisted of miniaturized matrix solid phase dispersion (micro-MSPD) in a glass Pasteur pipette using Captiva EMR (enhanced matrix removal)-lipid as the sorbent. Good linearity was obtained in the work range (1-500 μg L-1) with a correlation coefficient (R2) ≥ 0.998. The method accuracy and precision were determined at two concentration levels. The results show recoveries ranging from 55 to 111%. The precision varied from 1.95 to 11.4% (RSD). The whole quantification limits were between 0.056 and 3.42 μg per kg dry weight. The analytical procedure was applied for the analyses of five mussel samples collected from Galician Rias. The major compound was BPA, and wild mussels from Rías de Ferrol, Vigo and A Coruña had the highest levels. The proposed method is suitable for the analysis of BPA and its analogues in mussel samples.
