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Formulation and Characterization of Triamcinolone Acetonide Acetate-Loaded Microspheres Prepared by a Static Mixing
Huan-Huan Du1,2, Li-Rong Wang1, Xin-Hong Wu1
1College of Pharmaceutical Sciences, Soochow University, Suzhou, People's Republic of China.
Current Drug Delivery
|June 7, 2024
Summary
This study developed Triamcinolone Acetonide Acetate (TAA) microspheres using a novel static mixing method with poly(lactic-co-glycolic acid) (PLGA). The optimized microspheres show enhanced encapsulation efficiency and sustained drug release over 28 days.
Area of Science:
- Biomaterials Science
- Pharmaceutical Technology
- Drug Delivery Systems
Background:
- Spray drying of microspheres faces reproducibility and scale-up challenges.
- Biodegradable microspheres are crucial for sustained drug delivery.
- Poly(lactic-co-glycolic acid) (PLGA) is a widely used carrier for controlled release applications.
Purpose of the Study:
- To develop Triamcinolone Acetonide Acetate (TAA) loaded biodegradable microspheres using a novel static mixing technique.
- To optimize the formulation and preparation conditions for TAA-loaded microspheres (TAA-MSs) for improved drug loading and encapsulation efficiency.
- To evaluate the physicochemical properties and in vitro release profile of the developed TAA-MSs.
Main Methods:
- TAA-MSs were prepared using a static mixing technique with PLGA as the sustained-release carrier.
- Optimization of PLGA concentration, polyvinyl alcohol (PVA) concentration, and phase ratios (oil/water, water/solidification).
- Characterization included Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared Spectroscopy (FT-IR).
- In vitro drug release studies were conducted using a water-bath vibration method at pH 7.4 and 37°C.
Main Results:
- Optimized TAA-MSs (1% PLGA, 1:3 oil/water, 1:2 water/solidification) yielded spherical particles (30-70 μm) with high drug loading (27.09%) and encapsulation efficiency (98.67%).
- XRD indicated a partial conversion of crystalline TAA to an amorphous form within the microspheres.
- DSC and FT-IR confirmed interactions between TAA and PLGA, indicating successful drug encapsulation.
- In vitro release studies demonstrated a significant sustained release profile, with only 20% drug released over 28 days.
Conclusions:
- The static mixing technique is effective for preparing TAA-loaded PLGA microspheres.
- The developed microspheres exhibit enhanced encapsulation efficiency and a sustained-release manner.
- This novel method offers a promising alternative for reproducible and scalable production of drug-loaded microspheres.
