A sensitive and simple method for determination of IgM in cerebrospinal fluid by a solid-phase enzyme-immunoassay....

A sensitive and simple solid-phase enzyme-immunoassay for quantitative determination of IgM in cerebrospinal fluid has been developed. This method is based on a sandwich technique and is much more sensitive than the competition enzyme-immunoassay which we described previously. The IgM values obtained by this sandwich technique were well correlated with those by the competition technique. This new method is considered to be more suitable for the estimation of IgM in cerebrospinal fluid because of its simplicity and good sensitivity.