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Related Experiment Video

Updated: Jun 24, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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linc01152 Regulates Cell Viability, Cell Migration and Cell Invasion of Breast Cancer via Regulating miR-320a and

Zhiwei Zhu1,2, Siting Lin3, Lihong Pang4,5

  • 1School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, No 138 Xianlin Avenue, Qixia District, Nanjing, 210023, China. zhuzhiwei1116@126.com.

Biochemical Genetics
|June 7, 2024
PubMed
Summary

This study reveals that the long non-coding RNA linc01152 promotes breast cancer progression by regulating the miR-320a-MTDH pathway. Inhibiting linc01152 reduces cancer cell viability, migration, and invasion.

Keywords:
Breast cancerCell invasionCell migrationCell viabilityLinc01152

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of cancer deaths globally.
  • Long non-coding RNAs (lncRNAs) play crucial roles in biological processes, including cancer development.
  • Understanding the specific functions of lncRNAs like linc01152 in breast cancer is vital for identifying new therapeutic targets.

Purpose of the Study:

  • To investigate the functional role of linc01152 in breast cancer.
  • To elucidate the regulatory mechanisms and downstream targets of linc01152 in breast cancer pathogenesis.
  • To validate the linc01152-miR-320a-MTDH regulatory network.

Main Methods:

  • Quantitative real-time PCR (RT-PCR) for linc01152 expression analysis.
  • siRNA-mediated inhibition of linc01152 expression.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays for cell viability, migration, and invasion.
  • Luciferase reporter assays to validate miRNA-mRNA interactions.

Main Results:

  • linc01152 expression was significantly upregulated in breast cancer cells compared to normal cells, with highest levels in BT474 and MDA-MB-468 cell lines.
  • Inhibition of linc01152 suppressed breast cancer cell viability, migration, and invasion.
  • The regulatory axis linc01152-miR-320a-MTDH was confirmed, and inhibition of miR-320a partially reversed the anti-cancer effects of linc01152 knockdown.

Conclusions:

  • The linc01152-miR-320a-MTDH regulatory network is implicated in breast cancer development and progression.
  • linc01152 acts as a potential oncogene in breast cancer, making it a promising target for therapeutic intervention.