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Utilization of low-stability variants in protein evolutionary engineering

Mitsutoshi Wakisaka1, Shun-Ichi Tanaka1, Kazufumi Takano1

  • 1Department of Biomolecular Chemistry, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan.

International Journal of Biological Macromolecules
|June 7, 2024
PubMed
Summary

Evolutionary engineering can be enhanced by including low-stability variants. Restabilizing these variants can lead to improved protein activity and diversified evolutionary pathways.

Keywords:
Evolutionary engineeringProtein activityProtein stability

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