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Related Concept Videos

Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

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Related Experiment Video

Updated: Jul 27, 2026

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
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Biomimetic Multilayered Lipid Nanovesicles for Potent Protein Vaccination.

Bon Il Koo1, Dong Jae Lee1, Rafia Tasnim Rahman1

  • 1Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Advanced Healthcare Materials
|June 7, 2024
PubMed
Summary

New ceramide-based lipid nanovesicles offer enhanced stability for vaccines. These robust multilamellar vesicles improve in vivo antigen delivery and immune responses, presenting a promising nanovaccine platform.

Keywords:
1‐O‐acylceramideadjuvantsantigensmultilamellar vesiclesvaccine delivery

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Area of Science:

  • Biotechnology
  • Materials Science
  • Immunology

Background:

  • Lipid vesicles are crucial for drug and gene delivery but suffer from poor in vivo stability.
  • Current stabilization methods involve chemical modifications with unclear metabolic fates.
  • A need exists for novel, non-chemically altered lipid vesicle stabilization techniques.

Purpose of the Study:

  • To develop a bio-mimetic approach for fabricating robust multilamellar lipid vesicles.
  • To enhance the in vivo delivery and stabilization of protein antigens using a novel nanovaccine platform.
  • To investigate the efficacy of ceramide-induced lipid vesicles for vaccine applications.

Main Methods:

  • Utilized 1-O-acylceramide, a natural skin lipid, to promote self-assembly of lipid nanovesicles.
  • Incorporated ceramide to anchor lipid bilayers, mimicking stratum corneum structure for enhanced stability.
  • Encapsulated ovalbumin (antigen) and monophosphoryl lipid A (adjuvant) within the vesicles.

Main Results:

  • Ceramide-induced vesicles demonstrated excellent stability against environmental stresses like freeze-thaw cycles.
  • Both unilamellar and multilamellar vesicles enhanced immune responses in vitro.
  • Multilamellar vesicles showed superior in vivo performance, inducing higher antibody and cytokine levels.

Conclusions:

  • Ceramide-induced multilamellar lipid vesicles represent a stable and effective nanovaccine platform.
  • This approach enhances antigen delivery and stability for improved vaccine efficacy.
  • The bio-mimetic strategy offers a promising alternative to chemically modified lipid vesicles.