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Updated: Jun 24, 2025
12:22
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
21.1K
Mitochondrial ribosome biogenesis and redox sensing
Michele Brischigliaro1, Ana Sierra-Magro1, Ahram Ahn2
1Department of Neurology, University of Miami Miller School of Medicine, FL, USA.
FEBS Open Bio
|June 7, 2024
Summary
Mitoribosome biogenesis involves mitochondrial RNA and nuclear proteins, guided by assembly factors. Recent studies highlight iron-sulfur clusters and redox-sensitive cysteines in regulating mitochondrial translation.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Mitoribosome biogenesis is crucial for mitochondrial protein synthesis.
- It involves mitochondrial RNA and nuclear-encoded proteins, coordinated by assembly factors.
Purpose of the Study:
- To outline current understanding of mammalian mitoribosome biogenesis.
- To highlight recent findings on iron-sulfur clusters and redox-sensitive cysteines.
Main Methods:
- Biochemical studies
- Cryo-electron microscopy (cryo-EM) of mammalian mitoribosomes
Main Results:
- Iron-sulfur clusters identified as structural components and in assembly factors.
- Redox-sensitive cysteines found in mitoribosome proteins and assembly factors.
Conclusions:
- Mitoribosome assembly is complex, involving coordinated RNA processing and protein association.
- Iron-sulfur clusters and redox-sensitive cysteines may act as redox sensors regulating mitochondrial translation under stress.
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