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RcOST1L phosphorylates RcPIF4 for proteasomal degradation to promote flowering in rose
Jingjing Sun1, Hongchi Liu1, Weinan Wang1
1Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of State Forestry and Grassland Administration on Biology of Ornamental Plants in East China, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
The New Phytologist
|June 7, 2024
Summary
Rose plants flower later in low light due to PHYTOCHROME-INTERACTING FACTORs (RcPIFs) stability. High light accelerates flowering by promoting RcPIF degradation via RcOST1L and RcphyB.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agronomy
Background:
- Flowering time is crucial for ornamental plants, but the molecular control of rose flowering under varying light intensity is not fully understood.
- Rose flowering is photoperiod insensitive and primarily influenced by light intensity.
Purpose of the Study:
- To elucidate the molecular mechanism regulating rose flowering time under different light intensities.
- To identify key proteins and pathways involved in light-mediated flowering control in roses.
Main Methods:
- Yeast two-hybrid screening to identify protein interactions.
- Phosphorylation assays to determine protein modification.
- Analysis of protein stability and localization under varying light conditions.
- Genetic analysis of phytochrome B (RcphyB) and OPEN STOMATA 1-Like (RcOST1L) in flowering regulation.
Main Results:
- Rose plants exhibit delayed flowering under low-light (LL) compared to high-light (HL) conditions.
- High light triggers phosphorylation and subsequent degradation of PHYTOCHROME-INTERACTING FACTORs (RcPIFs).
- RcOST1L kinase interacts with and phosphorylates RcPIF4 at serine 198, promoting its degradation under HL.
- RcphyB enhances RcOST1L-mediated RcPIF4 phosphorylation and nuclear accumulation, accelerating flowering under HL.
Conclusions:
- RcPIF stability, regulated by RcOST1L and RcphyB, is a key determinant of rose flowering time under varying light intensities.
- The RcphyB-RcOST1L-RcPIF4 module plays a critical role in light-mediated flowering control in roses.
- Understanding this pathway provides insights into optimizing flowering in ornamental plants.
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