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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Capillary Electrophoresis: Instrumentation01:20

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Related Experiment Video

Updated: Jun 24, 2025

High Throughput Single-cell and Multiple-cell Micro-encapsulation
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Superimposed Electric Field Enhanced Electrospray for High-Throughput and Consistent Cell Encapsulation.

Zejun Fan1,2, Yihan Chen1, Zhen Yang3,4

  • 1School of Biomedical Engineering, Tsinghua Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.

Advanced Healthcare Materials
|June 8, 2024
PubMed
Summary

A new superimposed electric field (SEF) electrospray method enhances cell encapsulation stability and efficiency. This advanced technique significantly improves induced pluripotent stem cell expansion and liver capsule function for biomedical applications.

Keywords:
cell encapsulationelectrosprayiPSC capsulemicrotissue engineering

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Materials Science

Background:

  • Existing microfluidic and electrospray methods for cell encapsulation face challenges with cell damage, instability, and capsule breakage.
  • High-throughput cell encapsulation requires methods that are both precise and gentle to maintain cell viability.

Purpose of the Study:

  • To introduce a novel superimposed electric field (SEF)-enhanced electrospray method for improved cell encapsulation.
  • To analyze the stability and efficiency of the SEF technique for producing core-shell capsules.
  • To demonstrate the potential of SEF-enhanced electrospray in key biomedical applications.

Main Methods:

  • Development and application of a superimposed electric field (SEF) electrospray system for cell encapsulation.
  • Quantitative stability analysis of the electrospray using stiffness theory under conical confinement.
  • Assessment of capsule production rate and cell viability in an aqueous environment.
  • Evaluation of SEF-enhanced encapsulation for induced pluripotent stem cell (iPSC) expansion and liver capsule development for disease modeling.

Main Results:

  • The SEF technique significantly enhances electrospray stability and biocompatibility, enabling rapid, continuous production of approximately 300 core-shell capsules per second.
  • Encapsulated human-derived induced pluripotent stem cells (iPSCs) showed a 92-fold increase in expansion over 10 days, maintaining pluripotency superior to 2D cultures.
  • Developed liver capsules demonstrated normal function and biomimetic lipid accumulation, suitable for steatosis modeling.

Conclusions:

  • The SEF-enhanced electrospray method represents a significant advancement in cell encapsulation technology.
  • This method offers a more efficient, stable, and biocompatible approach for various biomedical applications, including clinical transplantation, drug screening, and cell therapy.