Related Experiment Video
Updated: Jun 24, 2025
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models
Published on: May 3, 2017
Aptamer-based electrochemical nanobiosensor for research and monitoring of multiple sclerosis in mice models
1Faculty of Pharmacy, Department of Analytical Chemistry, Ege University, 35100 Izmir, Bornova, Turkey; Graduate School of Natural and Applied Sciences, Department of Biomedical Technologies, Ege University, 35100 Izmir, Bornova, Turkey.
Abstract:
Multiple sclerosis (MS) is a severe progressive autoimmune-inflammatory, demyelinating process in the central nervous system (CNS) with heterogeneous neurological symptoms appearing as a consequence of myelin break down. Myelin basic protein (MBP) makes up to 30 % of the CNS myelin [1] and it is known to be released into the cerebrospinal fluid (CSF) as a bioindicator of MS. Autoimmune encephalomyelitis (EAE) is a mice model of MS widely used for research and development of new treatments [2]. Herein, MBP specific aptamer developed for possible therapeutic purposes in mouse model [3] was applied as a bioreceptor for MBP recognition. A nanobiosensor for MBP detection and monitoring was developed by using graphene oxide (GO) nanoparticles integrated onto the screen-printed carbon electrodes (SPCE) and aptamer immobilized to create a bioactive layer on the sensor surface for MBP binding. The measurements were carried out using electrochemical impedance spectrometry (EIS). Validation studies were carried out in a biological matrix (artificial CSF) containing MBP, and MSA. The aptasensor had LOD in artificial CSF 0.01 ng/mL and showed its usability in the concentration range of 0.01 … 64 ng/mL.
Insights
A novel aptasensor detects myelin basic protein (MBP), a biomarker for multiple sclerosis (MS), in cerebrospinal fluid (CSF). This graphene oxide-based biosensor offers sensitive and specific MBP detection for MS monitoring.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Analytical Chemistry
Background:
- Multiple sclerosis (MS) is a progressive autoimmune disease affecting the central nervous system (CNS), characterized by myelin breakdown.
- Myelin basic protein (MBP) is a key component of CNS myelin and its presence in cerebrospinal fluid (CSF) indicates MS.
- The development of sensitive detection methods for MBP is crucial for MS diagnosis and monitoring.
Purpose of the Study:
- To develop a novel nanobiosensor for the detection and monitoring of MBP.
- To utilize an MBP-specific aptamer as a bioreceptor integrated with graphene oxide nanoparticles.
- To validate the aptasensor's performance in a biological matrix for potential MS diagnostics.
Main Methods:
- Fabrication of a nanobiosensor using graphene oxide (GO) nanoparticles on screen-printed carbon electrodes (SPCE).
- Immobilization of an MBP-specific aptamer onto the GO-modified electrode surface to create a bioactive layer.
- Electrochemical impedance spectrometry (EIS) was employed for MBP detection and quantification.
Main Results:
- The aptasensor demonstrated high sensitivity for MBP detection with a limit of detection (LOD) of 0.01 ng/mL in artificial CSF.
- The sensor showed reliable performance within a concentration range of 0.01–64 ng/mL.
- Validation studies confirmed the aptasensor's usability in a biological matrix (artificial CSF) containing MBP and multiple sclerosis-associated autoantibodies (MSA).
Conclusions:
- A novel aptasensor based on graphene oxide and aptamers has been successfully developed for MBP detection.
- The developed aptasensor shows significant potential as a sensitive and specific diagnostic tool for multiple sclerosis monitoring.
- This nanobiosensor offers a promising platform for early detection and management of MS through MBP biomarker analysis.
