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A double-negative feedback loop mediated by non-coding RNAs contributes to tooth morphogenesis.

Meng Sun1, Na Li2, Weixing Zhang2

  • 1Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710049, China; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China.

Cells & Development
|June 9, 2024
PubMed
Summary
This summary is machine-generated.

A novel double-negative feedback loop involving long non-coding RNA LOC102159588 and microRNA miR-133b regulates tooth development in miniature swine. This intricate mechanism ensures normal tooth morphogenesis by controlling apoptosis, preventing malformations.

Keywords:
Double-negative feedback loopNon-coding RNAsTooth morphogenesis

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Tooth morphogenesis is a complex process regulated by signaling factors.
  • Non-coding RNAs play crucial roles in fine-tuning developmental signaling pathways.

Purpose of the Study:

  • To elucidate the role of a long non-coding RNA (LOC102159588) and microRNA (miR-133b) in miniature swine tooth morphogenesis.
  • To investigate the regulatory mechanism of a double-negative feedback loop between LOC102159588 and miR-133b.

Main Methods:

  • Investigated the interaction between LOC102159588 and miR-133b in miniature swine tooth development.
  • Analyzed the regulatory effects of miR-133b on LOC102159588 transcription via Sp1.
  • Examined the role of LOC102159588 in regulating pre-miR-133b nuclear export and cytoplasmic sponging via exportin-5.

Main Results:

  • Identified a double-negative feedback loop between LOC102159588 and miR-133b.
  • Demonstrated that miR-133b represses LOC102159588 transcription through Sp1.
  • Showed that LOC102159588 inhibits miR-133b transport and function, creating a feedback loop.
  • This loop modulates endogenous apoptosis to maintain normal tooth morphogenesis.

Conclusions:

  • The discovered double-negative feedback loop is essential for normal tooth morphogenesis in miniature swine.
  • Disruptions in this regulatory loop can lead to arrested tooth development and malformations.
  • This finding highlights the critical role of non-coding RNA interactions in craniofacial development.