Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical Safety Assessment of the HB-ATV-8 Intranasal Vaccine Designed to Control Atherosclerosis and Metabolic-Associated Fatty Liver Disease.

Archives of medical research·2026
Same author

Enhancing Cytosolic Internalization of [<sup>177</sup>Lu]Lu-iPSMA in Prostate Cancer Cells: The Effect of Conjugating a GRP78 Inhibitor to the Radiotherapeutic Molecule.

International journal of molecular sciences·2025
Same author

Inhibition of the oncogenic channel Kv10.1 by the antipsychotic drug penfluridol.

Frontiers in pharmacology·2025
Same author

Altered Expression of Thyroid- and Calcium Ion Channels-Related Genes in Rat Testes by Short-Term Exposure to Commercial Herbicides Paraquat or 2,4-D.

Journal of xenobiotics·2024
Same author

The Influence of Sex Steroid Hormone Fluctuations on Capsaicin-Induced Pain and TRPV1 Expression.

International journal of molecular sciences·2024
Same author

Fluorescent membrane potential assay for drug screening on Kv10.1 channel: identification of BL-1249 as a channel activator.

Frontiers in pharmacology·2023
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

15.1K

Cell-Based Thallium-Influx Fluorescence Assay for Kv10.1 Channels.

María Luisa Durán-Pastén1, Enoch Luis2

  • 1Laboratorio Nacional de Canalopatías, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México City, Mexico.

Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2024
PubMed
Summary
This summary is machine-generated.

This study optimized a cell-based assay using thallium influx to measure potassium channel Kv10.1 activity. This method aids in discovering new modulators for these important drug targets.

Keywords:
CancerCell-based fluorescenceKv10.1Microplate readerOncochannelThallium influxVoltage-gated ion channels

More Related Videos

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

2.1K
A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
05:31

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

Published on: April 24, 2012

14.4K

Related Experiment Videos

Last Updated: Jun 24, 2025

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

15.1K
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

2.1K
A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
05:31

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

Published on: April 24, 2012

14.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Cell-based fluorescent assays are crucial for identifying ion channel modulators.
  • Potassium (K+) channels are a diverse and significant class of ion channels, making them key targets for drug discovery.
  • Existing assays utilize thallium (Tl+) sensitive dyes to detect K+ channel activity.

Purpose of the Study:

  • To optimize the FLIPR Potassium Assay Kit for measuring Kv10.1 channel activity.
  • To validate a thallium influx-based assay for ion channel research.

Main Methods:

  • Utilized a thallium-sensitive fluorescent dye.
  • Measured thallium (Tl+) influx through Kv10.1 channels.
  • Optimized the FLIPR Potassium Assay Kit protocol.

Main Results:

  • Successfully optimized the FLIPR Potassium Assay Kit.
  • Demonstrated the assay's capability to measure Kv10.1 activity via thallium influx.
  • Established a robust method for ion channel modulator screening.

Conclusions:

  • The optimized thallium influx assay is effective for assessing Kv10.1 channel function.
  • This assay provides a valuable tool for the discovery of Kv10.1 modulators.
  • Enhances the utility of cell-based assays in ion channel drug discovery.