Cell-Based Thallium-Influx Fluorescence Assay for Kv10.1 Channels

The development of cell-based fluorescent assays has resulted in an incredible tool for searching new ion channels' modulators with a biophysical and clinical profile. Among all the ion channels, potassium (K⁺)-permeable channels represent the most diverse and relevant for cell function, making them attractive targets for drug discovery. Some of the cell-based assays for K⁺ channels take advantage of a thallium-sensitive dye whose fluorescence increased upon the binding of thallium (Tl⁺), an ion able to move through K⁺ channels. We optimize the FLIPR Potassium Assay Kit based on thallium influx to measure the Kv10.1 activity.