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A rapid protocol for inducing acute pancreatitis in zebrafish models
Kívia Vanessa Gomes Falcão1, Rafael David Souto de Azevedo2, Luiza Rayanna Amorim de Lima3
1Departamento de Bioquímica, Universidade Federal de Pernambuco - UFPE. Brazil.
Summary
Researchers developed a new zebrafish model for acute pancreatitis (AP) using caerulein injections. This standardized model mimics human AP responses, aiding in the development of new treatments.
Area of Science:
- Comparative physiology
- Animal models
Background:
- Acute pancreatitis (AP) is an inflammatory pancreatic disorder.
- Rodent models of AP show limitations due to divergent pathophysiological responses compared to humans.
Purpose of the Study:
- To standardize and validate a novel acute pancreatitis (AP) model in zebrafish.
- To establish a more relevant preclinical model for AP research.
Main Methods:
- Induction of AP in zebrafish via intraperitoneal injections of synthetic caerulein (10 μg/kg, four times).
- Detailed protocol for solution preparation, injection, and monitoring.
- Validation through biochemical and histological analyses of pancreatic tissue.
Main Results:
- Histological analysis confirmed necrosis within 12 hours post-injection.
- Observed extracellular zymogen granules, consistent with human and rodent AP models.
- Successfully standardized and validated the zebrafish AP model.
Conclusions:
- The zebrafish AP model offers a standardized and validated platform for AP research.
- This model can accelerate preclinical and clinical studies for novel AP therapeutics.
- Expands research tools for developing faster, more effective AP prevention and treatment strategies.
