Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

M13 clones carrying point mutations: identification by solution hybridization.

A N Hobden, M J Read, C W Dykes

    Analytical Biochemistry
    |January 1, 1985
    PubMed
    Summary

    This study introduces a rapid solution hybridization method for identifying M13 clones with specific DNA sequences. The technique accurately detects single-base differences, aiding in the identification of mutant sequences from oligonucleotide-directed mutagenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A process mining- deep learning approach to predict survival in a cohort of hospitalized COVID-19 patients.

    BMC medical informatics and decision making·2022
    Same author

    Impact of the Covid pandemic on health care equity in NHS General Dental Practice.

    Community dental health·2022
    Same author

    Guidelines relevant to paediatric dentistry - do foundation dentists and general dental practitioners follow them? Part 2: Treatment and recall.

    British dental journal·2018
    Same author

    Guidelines relevant to paediatric dentistry - do foundation dentists and general dental practitioners follow them? Part 1: diagnosis and prevention.

    British dental journal·2018
    Same author

    The integration of functional and fixed appliance treatment.

    Journal of orthodontics·2001
    Same author

    The effectiveness and efficiency of hygienists in carrying out orthodontic auxiliary procedures.

    British journal of orthodontics·1999

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • Accurate identification of specific DNA sequences in M13 clones is crucial for molecular biology research.
    • Oligonucleotide-directed mutagenesis requires precise methods for verifying introduced mutations.

    Purpose of the Study:

    • To develop a rapid and efficient solution hybridization procedure for identifying M13 clones containing specific DNA sequences.
    • To enable the discrimination of sequences differing by a single nucleotide base.

    Main Methods:

    • Utilized a radiolabeled oligonucleotide probe for hybridization with M13 phage-containing supernatant from Escherichia coli cultures.
    • Employed sodium dodecyl sulfate during incubation and agarose gel electrophoresis for separation.
    • Detected hybridization through autoradiography.

    Related Experiment Videos

    Main Results:

    • The solution hybridization method rapidly identifies M13 clones with target sequences.
    • The procedure can distinguish between sequences with single-base variations.
    • Demonstrated utility in identifying mutant sequences generated by oligonucleotide-directed mutagenesis.

    Conclusions:

    • The described solution hybridization technique offers a faster and more convenient alternative to traditional membrane hybridization.
    • This method allows for the use of multiple probes on a single gel, increasing efficiency.
    • The procedure is effective for identifying specific sequences and single-nucleotide polymorphisms in M13 clones.