Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fracture Behavior and Energy Conversion of Concrete-Rock Composites Subjected to Fatigue Disturbance: Experimental and Numerical Approaches.

Materials (Basel, Switzerland)·2026

Same author

An asynchronous production line of meiotic prophase I in the mouse fetal ovary.

Experimental cell research·2026

Same author

Closed-loop high-precision two-photon lithography based on a multiplexed single-cavity dual-comb laser.

Nature communications·2026

Same author

A Wearable Electrochemical Sensing Platform for Rapid Detection of Organophosphorus Pesticides: A Flexible Biosensor Based on Screen-Printed Electrodes and Organophosphorus Hydrolase.

Sensors (Basel, Switzerland)·2026

Same author

Model-Driven Deep Learning Enables Speckle-Free Holography for 3D Parallel Nanofabrication.

Research (Washington, D.C.)·2026

Same author

The whole-course of supervised home-based multi-modal prehabilitation to improve clinical outcome in patients undergoing neoadjuvant chemotherapy prior to gastrectomy: a single-center randomized controlled trial.

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·2026

Search research articles

Related Experiment Video

Updated: Jun 24, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Compressive sensing-based multi-focus line-scanning two-photon microscopy for fast 3D imaging.

Gan Liu, Bingxu Chen, Zezhi Tian

|June 11, 2024

Summary

This summary is machine-generated.

This study introduces a novel multi-focus microscope for rapid 3D biological imaging. The advanced system achieves high-speed volume imaging, enabling detailed visualization of neural networks and live animal studies.

More Related Videos

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

Related Experiment Videos

Last Updated: Jun 24, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

Area of Science:

Biophysics
Optical Imaging
Microscopy

Background:

Fast 3D volume imaging is crucial for advancing biological studies.
Existing methods face limitations in speed and resolution for deep tissue visualization.

Purpose of the Study:

To design and characterize a multi-focus line-scanning two-photon microscope.
To achieve high-speed 3D volume imaging for biological samples.

Main Methods:

Utilized a digital micromirror device (DMD) for multi-focus generation via binary holography.
Employed a galvanometric mirror for scanning the focus array across the imaging volume.
Integrated compressive sensing algorithms for sparse samples.

Main Results:

Achieved a volume imaging rate of 15.5 volumes/sec over 77 × 120 × 40 µm³ for sparse samples.
Generated high-resolution optical cross-sections at 107 frames/sec.
Demonstrated versatility with imaging of microbeads, pollens, and mouse brain slices.

Conclusions:

The multi-focus line-scanning microscope offers a fast and versatile platform for 3D imaging.
Suitable for deep tissue imaging and dynamic live animal studies.
Potential to significantly enhance biological research requiring high-speed volumetric data.