Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harmane induces apoptosis through RRM2B and suppresses colorectal cancer progression.

mSystems·2026
Same author

Response to Letter regarding "Concerns regarding the applicability of ventilator-associated pneumonia prediction models".

American journal of infection control·2026
Same author

Hepatic SNHG9 links gut microbiota to liver protection in drug-induced liver injury.

Nature communications·2026
Same author

Gaussian-Fitting-Enabled High-Accuracy pH Detection for Light-Addressable Potentiometric Sensor.

Sensors (Basel, Switzerland)·2026
Same author

Optical cross-purity.

Optics letters·2026
Same author

The relationship between social interaction patterns and cognitive trajectory in older adult: evidence from ELSA.

BMC public health·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

Author Spotlight: Advancing 3D Modeling for Enhanced Diagnosis and Treatment of Pulmonary Nodules in Early-Stage Lung Cancer
07:53

Author Spotlight: Advancing 3D Modeling for Enhanced Diagnosis and Treatment of Pulmonary Nodules in Early-Stage Lung Cancer

Published on: October 13, 2023

1.4K

Three-dimensional tomographic reconstruction using Voronoi weighting.

John A B Aziz, Seth Smith-Dryden, Bahaa E A Saleh

    Optics Express
    |June 11, 2024
    PubMed
    Summary
    This summary is machine-generated.

    Selecting appropriate reconstruction weights is crucial for 3D tomographic reconstruction, especially with limited illumination angles. Voronoi weighting significantly enhances the accuracy of optical diffraction tomography.

    More Related Videos

    3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
    07:01

    3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

    Published on: October 24, 2019

    9.8K
    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
    13:43

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

    Published on: June 24, 2013

    14.0K

    Related Experiment Videos

    Last Updated: Jun 24, 2025

    Author Spotlight: Advancing 3D Modeling for Enhanced Diagnosis and Treatment of Pulmonary Nodules in Early-Stage Lung Cancer
    07:53

    Author Spotlight: Advancing 3D Modeling for Enhanced Diagnosis and Treatment of Pulmonary Nodules in Early-Stage Lung Cancer

    Published on: October 13, 2023

    1.4K
    3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
    07:01

    3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

    Published on: October 24, 2019

    9.8K
    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
    13:43

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

    Published on: June 24, 2013

    14.0K

    Area of Science:

    • Optics
    • Image Reconstruction
    • Computational Imaging

    Background:

    • Three-dimensional tomographic reconstruction is vital in various scientific fields.
    • Accurate reconstruction often depends on the selection of illumination angles.
    • Nonuniform angular sampling presents challenges in achieving high-fidelity reconstructions.

    Purpose of the Study:

    • To investigate methods for improving 3D tomographic reconstruction fidelity under measurement constraints.
    • To evaluate the effectiveness of reconstruction weighting strategies for nonuniform angular distributions.
    • To demonstrate the benefits of Voronoi weighting in optical diffraction tomography.

    Main Methods:

    • Utilized optical diffraction tomography (ODT) principles.
    • Implemented and compared different reconstruction weighting schemes.
    • Focused on scenarios with nonuniformly distributed illumination angles.
    • Applied Voronoi weighting as a key method.

    Main Results:

    • Demonstrated that Voronoi weighting significantly improves reconstruction fidelity.
    • Showcased enhanced accuracy in 3D tomographic reconstructions with limited angular data.
    • Quantified the improvement in fidelity compared to other weighting methods.

    Conclusions:

    • Voronoi weighting is a highly effective strategy for enhancing optical diffraction tomography.
    • Appropriate selection of reconstruction weights is critical for accurate 3D imaging under constraints.
    • This method offers a significant advancement for 3D tomographic reconstruction applications.