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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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DNA-Programmed Four-Bit Quaternary Fluorescence Encoding (FLUCO) Enables 51-Colored Bioimaging Analysis.

Dongsheng Mao¹, Xiaochen Tang², Runchi Zhang^1,3

¹Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P. R. China.

Journal of the American Chemical Society

|June 11, 2024

Summary

Researchers developed fluorescence encoding (FLUCO), a DNA self-assembly system for molecular-level color encoding. This technology enables accurate, target-responsive color generation for advanced biological imaging and analysis.

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Area of Science:

Molecular Biology
Biotechnology
Computer Science

Background:

Accurate molecular-level color encoding is vital for fields like digital imaging and spectral analysis.
Current methods face challenges in achieving target-responsive and amplified color encoding.
Molecular encoding holds potential for revolutionizing scientific research and technological innovation.

Purpose of the Study:

To propose a novel multibit DNA self-assembly system for accurate, target-responsive color encoding at the molecular level.
To introduce fluorescence encoding (FLUCO) as a method for amplified molecular color representation.
To demonstrate the potential of FLUCO in advanced scientific applications.

Main Methods:

Development of a computer-aided design (CAD) based multibit DNA self-assembly system.
Establishment of a quaternary FLUCO system utilizing four-bit DNA self-assembly.
Modeling and validation of the color encoding capabilities of the FLUCO system.

Main Results:

The proposed FLUCO system enables accurate, target-responsive, and amplified color encoding at the molecular level.
A quaternary FLUCO system was established, capable of encoding 51 distinct colors.
FLUCO facilitates simultaneous imaging of multiple targets, overcoming conventional channel limitations.

Conclusions:

FLUCO represents a significant advancement in controllable molecular encoding.
This technology offers immense potential for spatial proteomic imaging and multitarget analysis.
FLUCO paves the way for innovations in spatial omics, cell analysis, and liquid biopsy.