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Updated: Jun 24, 2025

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
Regioselective and solvent-dependent photoisomerization induced internal conversion in red fluorescent protein
Basanta Kumar Rajbongshi1, Sheikh Abdullah1, Bittu Lama2
1Department of Chemistry, Cotton University Panbazar Guwahati Assam 781001 India basanta.rajbongshi@cottonuniversity.ac.in sheikha2607@gmail.com.
Abstract:
Photophysical properties of three red fluorescent protein (RFP) chromophore analogues are reported here. The three RFP chromophore analogues differ in the additional conjugation present in the RFP chromophore. The three chromophores do not exhibit any solvent effect in both absorption and fluorescence spectra. The photoirradiation experiments and recording of 1H NMR before and after irradiation on one of the three RFP model chromophores show isomerization of the (Z,E) diastereomer to the (E,E) diastereomer. Calculation of S0 and S1 potential energy curves shows the preference for isomerization through the exocyclic C[double bond, length as m-dash]C bond with Z-stereochemistry, thus corroborating the experimental results. The computational studies also suggest that torsional motion along the exocyclic C[double bond, length as m-dash]C bond pushes the molecules to a conical intersection, thus paving the pathway for radiationless deactivation.
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