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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Real-Time Detection of Multiple Intracellular MicroRNAs using an Ultrasound-Propelled Nanomotor-Based Dynamic

Li Li1, Keming Tan1, Yun Bai1

  • 1School of Public Health, School of Biomedical Engineering, Guangzhou Medical University, Guangzhou 511436, China.

Analytical Chemistry
|June 11, 2024
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This study introduces a novel ultrasound-powered nanomotor probe for rapid, real-time detection of multiple intracellular microRNAs (miRNAs). The dynamic fluorescent probe enables sensitive and specific identification of miRNAs within 15 minutes, distinguishing cancer cells from normal cells.

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Nanotechnology

Background:

  • Accurate detection of intracellular microRNAs (miRNAs) is crucial for disease diagnosis and management.
  • Real-time, multiplexed intracellular miRNA detection remains a significant challenge in current diagnostics.

Purpose of the Study:

  • To develop a novel ultrasound (US)-powered nanomotor-based dynamic fluorescent probe for real-time OFF-ON fluorescent determination of multiple intracellular miRNAs.
  • To enhance probe-target interactions for efficient and prompt hybridization using US-propelled nanomotors.

Main Methods:

  • Utilized multicolored quantum dot (QD)-labeled single-stranded DNA (ssDNA)/graphene oxide (GO)-coated US-powered gold nanowire (AuNW) nanomotors.
  • Employed a fluorescence quenching mechanism (GO) and OFF-ON switching upon target miRNA binding.
  • Leveraged ultrasound propulsion to enhance probe-target interactions and hybridization kinetics.

Main Results:

  • Achieved simultaneous quantitative analysis of miR-10b and miR-21 in vitro within 15 minutes with high sensitivity and specificity.
  • Demonstrated one-step real-time discrimination between A549 cancer cells and L02 normal cells using multicolor QDs.
  • Obtained results consistent with quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Conclusions:

  • The developed nanomotor-based dynamic fluorescent probe enables rapid, "on the move" specific detection of multiple intracellular miRNAs in intact cells.
  • Facilitates real-time monitoring of intracellular miRNA expression, offering potential for novel biodetection applications.
  • Presents a promising platform for advancing diagnostic tools in molecular biology and medicine.