Electrochemical and Plasmonic Detection of Myocardial Infarction Using Microfluidic Biochip Incorporated with...

Area of Science:

• Biomedical Engineering
• Nanomaterials Science
• Analytical Chemistry

Background:

• Early detection of cardiac biomarkers like cardiac myoglobin (cMb) and cardiac troponin I (cTnI) is crucial for diagnosing cardiovascular diseases.
• Existing detection methods often face limitations in sensitivity, speed, or complexity.

Purpose of the Study:

• To develop a microfluidic device for sensitive and rapid electrochemical and surface plasmon resonance (SPR) detection of cMb and cTnI.
• To investigate the performance of a novel nanostructured electrode material for enhanced biosensing capabilities.

Main Methods:

• Fabrication of a microfluidic device with mesoporous Ni3V2O8 nanoscaffolds grafted with reduced graphene oxide (rGO) on working electrodes.
• Utilized dual-modality sensing: electrochemical detection and surface plasmon resonance (SPR) spectroscopy.
• Quantified detection limits (LoD) and sensitivities for cMb and cTnI using standard solutions.

Main Results:

• Achieved low limits of detection (LoD) in the picogram per milliliter (pg/mL) range for both cMb and cTnI.
• Electrochemical detection showed LoDs of 2.0 pg/mL for cTnI and 4.7 pg/mL for cMb, with high sensitivities.
• SPR detection demonstrated LoDs of 8.8 pg/mL for cMb and 7.3 pg/mL for cTnI, complementing electrochemical data.

Conclusions:

• The dual-modality microfluidic sensor provides sensitive, rapid, and reliable detection of cardiac biomarkers.
• The combination of electrochemical and SPR modes offers self-verification and reduces false readings.
• This innovative sensor has significant potential for improving cardiovascular disease management and monitoring other biomolecules.