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Updated: Jun 24, 2025

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity
Aaron H Liepman1, Mara Jaworski2, Citlalxochilt Ramirez-Lopez2
1Biology Department, Eastern Michigan University, Ypsilanti, MI, USA. aliepman@emich.edu.
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Hydroxypyruvate reductase (HPR; EC 1.1.1.81) activity is integral to the photorespiratory pathway. Within photorespiration, HPR catalyzes the reduction of hydroxypyruvate, a product of the serine:glyoxylate aminotransferase reaction to glycerate, a substrate for glycerate kinase, using NADH as cofactor. Here we detail a spectrophotometric assay for measuring HPR activity in vitro by following the consumption of NADH at 340 nm.

