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Ultrabright NIR-IIb Fluorescence Quantum Dots for Targeted Imaging-Guided Surgery.

Hui Li1, Zhong Du2, Lijun Zhu2

  • 1Department of Epidemiology and Health Statistics, School of Public Health, Xinjiang Medical University, Urumqi 830054, China.

ACS Applied Materials & Interfaces
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Summary

New quantum dots (PbS@CdS@PEG NPs) enable precise, fluorescence-guided tumor surgery in the NIR-IIb window. This method minimizes damage to surrounding healthy tissue, improving surgical outcomes.

Keywords:
NIR-IIb fluorescence imagingPbS@CdS quantum dotimaging-guided surgeryin vivomPEG-SH

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Medical Imaging

Background:

  • Fluorescence-guided surgery offers precise tumor removal but faces challenges with low contrast at tumor margins and damage to healthy tissue.
  • Lead/cadmium sulfide quantum dots (PbS@CdS QDs) show promise for near-infrared (NIR-II) imaging but can lose quantum yield (QY) and fluorescence after hydrophilic coating, impacting biosafety and imaging quality.

Purpose of the Study:

  • To develop novel hydrophilic core-shell quantum dots (PbS@CdS@PEG NPs) for enhanced fluorescence-guided surgery.
  • To improve QY, NIR-IIb fluorescence, and biosafety of quantum dots for surgical navigation.
  • To reduce damage to healthy tissue during tumor resection.

Main Methods:

  • Synthesized hydrophilic core-shell PbS@CdS@PEG NPs with a small diameter (~8 nm) and NIR-IIb emission (~1600 nm).
  • Utilized mPEG-SH (MW: 2000) for hydrophobicity and enhanced biosafety of PbS@CdS QDs.
  • Performed *in vivo* fluorescence-guided cervical tumor resection using an aqueous solution of the NPs.

Main Results:

  • Achieved brilliant NIR-IIb emission at ~1600 nm with a high QY (~30.2%) and resistance to photobleaching.
  • Demonstrated successful *in vivo* fluorescence-guided cervical tumor resection immediately after NP injection.
  • Significantly reduced damage to normal tissues at tumor margins (100-500 μm thickness).

Conclusions:

  • Hydrophilic core-shell PbS@CdS@PEG NPs are effective for NIR-IIb fluorescence-guided surgery.
  • This approach enhances surgical precision by improving fluorescence contrast and minimizing collateral damage to healthy tissue.
  • The developed NPs offer a promising tool for safer and more effective tumor resection.