Related Experiment Video
Updated: Jun 24, 2025
12:49
Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
11.6K
Substrate and Functional Diversity of Protein Lysine Post-translational Modifications
Bingbing Hao1,2,3, Kaifeng Chen1,2, Linhui Zhai1,4,5
1State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Genomics, Proteomics & Bioinformatics
|June 11, 2024
Summary
Lysine post-translational modifications (PTMs) regulate protein functions in health and disease. This review covers lysine PTMs, their regulatory enzymes, proteomics advances, and therapeutic targets for disease treatment.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- Lysine post-translational modifications (PTMs) are crucial for diverse biological processes, regulating histone and non-histone proteins.
- Dysregulation of lysine PTMs is linked to various diseases, making their regulatory factors (writers, erasers, readers) therapeutic targets.
Purpose of the Study:
- To review diverse lysine PTMs, their regulatory enzymes, and advances in proteomics for PTM discovery.
- To discuss the biological functions and crosstalk of lysine PTMs on proteins.
- To highlight current druggable targets for disease therapy.
Main Methods:
- Review of current literature on lysine PTMs.
- Summary of mass spectrometry (MS) and antibody-based enrichment technologies for PTM analysis.
- Discussion of proteomics advances in lysine PTM characterization.
Main Results:
- Lysine PTMs play fundamental roles in cellular functions, and their dysregulation is disease-associated.
- MS and affinity enrichment technologies have significantly advanced PTM discovery and decoding.
- Crosstalks between lysine PTMs influence protein function and cellular mechanisms.
Conclusions:
- Global characterization of lysine PTMs is essential for understanding regulatory networks and disease mechanisms.
- Targeting lysine PTM regulatory factors offers promising therapeutic strategies for various diseases.
- Further research into PTM crosstalks and druggable targets will advance disease treatment.
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