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A Quantitative Glycomics and Proteomics Combined Purification Strategy

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Published on: March 8, 2016

Recent advances in microscale separation techniques for glycome analysis.

Chenchen Liu¹, Koji Otsuka^2,3, Takayuki Kawai^1,4

¹Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.

Journal of Separation Science

|June 12, 2024

Summary

Microscale separation technologies enhance glycomic analysis by offering high resolution and speed. This review explores advancements in these techniques for glycan analysis from 2014 to 2023.

Keywords:

capillary electrophoresis glycans glycome glycoprotein microchip electrophoresis nanoflow liquid chromatography

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Area of Science:

Glycomics
Analytical Chemistry
Biotechnology

Background:

Glycans and glycoconjugates are crucial in biological processes, including cellular interactions and organism structure.
Glycomic analysis utilizes liquid-phase separation for purification, separation, and identification of these molecules.
Microscale separation technologies offer advantages like high resolution, sensitivity, and speed for glycan analysis.

Purpose of the Study:

To review the fundamentals and applications of microscale separation technologies in glycomic analysis.
To cover advancements in microscale separation techniques for glycan analysis from 2014 to 2023.

Main Methods:

Review of liquid-phase separation technologies operating at scales <100 µm.
Focus on capillary electrophoresis, nanoflow liquid chromatography, and microchip electrophoresis.
Analysis of new strategies and applications in glycan analysis.

Main Results:

Microscale separation technologies are continuously evolving to meet glycan analysis challenges.
These technologies provide environmental friendliness, high resolution, sensitivity, and speed.
Significant progress in microscale separation strategies for glycan analysis has been made.

Conclusions:

Microscale separation technologies are vital for advancing glycomic analysis.
Continued innovation in these techniques promises further breakthroughs in understanding glycan functions.
The review highlights the impact of microscale separation on the field of glycomics.