Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Call For Papers: Molecular Understanding and Formulation Design for Peptide Delivery.

Molecular pharmaceutics·2026
Same author

Analyzing Spatial Variations in Molecular Mobility in Hydrated Amorphous Drug-Polymer Blends Using Fourier Transform Fluorescence Recovery After Photobleaching and Image Segmentation.

The journal of physical chemistry. B·2026
Same author

The expanding role of formulations to enable oral delivery of poorly water-soluble drugs.

Nature reviews. Drug discovery·2026
Same author

Addressing the Release and Permeation Challenges of High-<i>T</i><sub><i>g</i></sub> Drugs in Amorphous Solid Dispersions.

Molecular pharmaceutics·2026
Same author

Investigating the Relationship between In Vitro and In Vivo Performance: The Role of Drug Loading, Release Rate, and Surface Area.

Molecular pharmaceutics·2026
Same author

Role of Dissolution Medium pH in the Release Behavior of Basic Drug-Copovidone Amorphous Solid Dispersions.

Molecular pharmaceutics·2026
Same journal

Structural Hairpin Anchoring-Mediated TtAgo Activity Regulation for Programmable Biosensing.

Analytical chemistry·2026
Same journal

Digital Revitalization of a Legacy Linear Ion Trap System.

Analytical chemistry·2026
Same journal

An Interface-Regulated Electrochemical Biosensing Platform Based on the Cascade Amplification of Primer Exchange Reaction and CRISPR/Cas12a for Noninvasive Bladder Cancer Diagnosis.

Analytical chemistry·2026
Same journal

Spatially Resolved Diffusion NMR for Structurally Heterogeneous Materials.

Analytical chemistry·2026
Same journal

Direct Whole-Blood Multiplexing of Small Molecules via a Micelle-Enhanced Chemiluminescent Paper Sensor with Mesoporous Silica Membrane.

Analytical chemistry·2026
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

11.2K

Diffusion Mapping with Diffractive Optical Elements for Periodically Patterned Photobleaching.

Dustin M Harmon1, Ziyi Cao1, Alex M Sherman1

  • 1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.

Analytical Chemistry
|June 12, 2024
PubMed
Summary
This summary is machine-generated.

Fourier transform-fluorescence recovery after photobleaching (FT-FRAP) with diffractive optical elements (DOEs) enables distance-dependent diffusion analysis. This technique enhances signal-to-noise for precise molecular mobility quantification in various media.

More Related Videos

Patterning via Optical Saturable Transitions - Fabrication and Characterization
08:19

Patterning via Optical Saturable Transitions - Fabrication and Characterization

Published on: December 11, 2014

6.8K
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
11:58

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Published on: February 29, 2012

83.4K

Related Experiment Videos

Last Updated: Jun 24, 2025

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

11.2K
Patterning via Optical Saturable Transitions - Fabrication and Characterization
08:19

Patterning via Optical Saturable Transitions - Fabrication and Characterization

Published on: December 11, 2014

6.8K
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
11:58

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Published on: February 29, 2012

83.4K

Area of Science:

  • Biophysics
  • Materials Science
  • Analytical Chemistry

Background:

  • Fluorescence Recovery After Photobleaching (FRAP) is a key technique for measuring molecular mobility.
  • Traditional FRAP methods can be limited in throughput and the range of diffusion distances analyzed.
  • Diffractive Optical Elements (DOEs) offer novel ways to pattern light for advanced microscopy techniques.

Purpose of the Study:

  • To introduce and validate Fourier transform-based FRAP (FT-FRAP) utilizing DOEs for enhanced diffusion analysis.
  • To enable parallel, distance-dependent diffusion measurements in biologically relevant media.
  • To improve signal-to-noise ratio and throughput for FRAP experiments.

Main Methods:

  • Integration of DOEs to create multidot array patterns for parallel point-bleach FRAP measurements.
  • Analysis of spatial harmonics in the Fourier transform domain to extract diffusion recovery curves at specific distances.
  • Mathematical derivation of FT-FRAP principles for 2D periodic photobleaching patterns.
  • Retrofitting FT-FRAP into high-throughput instrumentation for automated analysis.

Main Results:

  • FT-FRAP successfully supports distance-dependent diffusion analysis in homogeneous and heterogeneous media.
  • The use of multidot arrays and Fourier transforms significantly improves signal-to-noise ratio.
  • The method allows for precise quantification of molecular mobility across a wide range of diffusion timescales (seconds to days).
  • Automated analysis of 96-well plates is demonstrated for high-throughput applications.

Conclusions:

  • FT-FRAP with DOEs is a powerful advancement for quantitative diffusion analysis.
  • The technique offers improved precision, throughput, and applicability to diverse samples.
  • This method opens new avenues for studying molecular dynamics in biological and material systems.