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Updated: Aug 17, 2026
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
Published on: February 22, 2016
Cell lineage evaluation in preimplantation mouse embryos by intracellular injection of IgG
The cell lineage in preimplantation mouse embryos was studied by injecting rabbit IgG into zygotes or into a blastomere of early or late 2-cell, 3- or 4-cell stage embryos. The localization of the IgG was detected by the peroxidase-antiperoxidase method. It was found that starting from the late 2-cell stage, lineage could be followed up to the cell populations of the blastocyst, inner cell mass and trophectoderm.
The cell lineage in preimplantation mouse embryos was studied by injecting rabbit IgG into zygotes or into a blastomere of early or late 2-cell, 3- or 4-cell stage embryos. The localization of the IgG was detected by the peroxidase-antiperoxidase method. It was found that starting from the late 2-cell stage, lineage could be followed up to the cell populations of the blastocyst, inner cell mass and trophectoderm.
