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Matrix-cytoskeletal interactions in the developing eye.

E D Hay

    Journal of Cellular Biochemistry
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Embryonic corneal epithelial cells differentiate in response to extracellular matrix (ECM) molecules. This interaction involves cell surface receptors and cytoskeletal organization, influencing cell behavior and differentiation.

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    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Biochemistry

    Background:

    • The embryonic avian corneal epithelium exhibits specific responses to extracellular matrix (ECM) molecules.
    • Cellular differentiation is influenced by cell shape and the surrounding ECM.
    • Epithelial and mesenchymal cells differ in their cell surface organization and interaction with the ECM.

    Purpose of the Study:

    • To investigate how embryonic corneal epithelial cells respond to ECM molecules in vitro.
    • To elucidate the role of cell surface receptors and cytoskeletal organization in ECM-mediated differentiation.
    • To explore the mechanisms underlying epithelial-mesenchymal transformations.

    Main Methods:

    • In vitro culture of embryonic avian corneal epithelium.

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  • Analysis of cellular responses to soluble and polymerized ECM molecules.
  • Investigation of cytoskeletal organization (actin, microtubules) and cell surface components (heparan sulfate proteoglycan, glycoprotein receptors).

    • Main Results:

    • Extracellular matrix molecules induce epithelial cell flattening, basal actin cytoskeleton organization, and a twofold increase in corneal stroma production.
    • Heparan sulfate proteoglycans and specific glycoprotein receptors for laminin and collagen are involved in organizing the epithelial cytoskeleton.
    • Epithelial cells cultured in collagen gels can exhibit mesenchymal characteristics, including pseudopodial extension and fibroblast-like behavior.

    Conclusions:

    • ECM-dependent cytoskeletal organization is crucial for enhancing corneal epithelial differentiation.
    • Cell surface receptors and cytoskeletal dynamics mediate the interaction between epithelial cells and the ECM.
    • In vitro manipulation can induce epithelial-mesenchymal transformations, offering insights into developmental processes.