Podoplanin depletion in tonsil-derived mesenchymal stem cells induces cellular senescence via regulation of the p16Ink4a/Rb pathway
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View abstract on PubMed
Summary
This summary is machine-generated.Podoplanin (PDPN) helps maintain stemness in tonsil-derived mesenchymal stem cells (TMSCs), reducing cellular senescence. PDPN levels decrease with passage, indicating its potential as a biomarker for MSC quality control in regenerative medicine.
Area Of Science
- Regenerative Medicine
- Stem Cell Biology
- Cellular Senescence
Background
- Mesenchymal stem cells (MSCs) are crucial for regenerative medicine but lose quality during in vitro expansion due to senescence.
- Cellular heterogeneity and acquired senescence in expanded MSCs limit their therapeutic efficacy.
- Investigating factors that maintain MSC stemness and mitigate senescence is critical for clinical applications.
Purpose Of The Study
- To investigate the role of podoplanin (PDPN) in minimizing cellular senescence.
- To determine if PDPN expression is associated with maintaining the stemness of tonsil-derived MSCs (TMSCs).
- To evaluate PDPN as a potential biomarker for MSC quality.
Main Methods
- Tonsil-derived MSCs (TMSCs) were isolated and cultured to early (3-7 passages) and late (>15 passages) stages.
- Cellular senescence and MSC characteristics were assessed in relation to passage number.
- PDPN expression was analyzed using fluorescence-activated cell sorting, and its function was studied using siRNA-mediated depletion.
Main Results
- Late-passaged, senescent TMSCs showed reduced proliferation, telomere length, pluripotency marker expression, and differentiation potential.
- PDPN protein levels decreased significantly with increased passage number.
- PDPN-positive cells retained stemness markers and differentiation capacity, while PDPN depletion accelerated senescence via the p16Ink4a/Rb pathway.
Conclusions
- PDPN expression is linked to the maintenance of stemness and reduced senescence in TMSCs.
- PDPN levels decrease in a passage-dependent manner, correlating with cellular aging.
- PDPN may serve as a valuable biomarker for assessing and mitigating cellular senescence in clinical MSC applications.
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