Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating Multi-View Features via Deep Generalized Canonical Correlation Analysis for Single-Cell Clustering.

International journal of molecular sciences·2026
Same author

Tuning Porphyrin-Based COFs for High Iodine Loading and Efficient Redox Kinetics in Aqueous Zinc-Iodine Batteries.

ACS applied materials & interfaces·2026
Same author

Multimodal 3D light-field and laser-speckle endoscopy.

bioRxiv : the preprint server for biology·2026
Same author

Repurposing primaquine diphosphate for imatinib-resistant chronic myeloid leukemia via targeting BCR-ABL and Wnt/β-catenin pathway.

iScience·2026
Same author

Dietary Supplement with Milk that Contains Different β-Caseins Influences Gut Microbiota and Serum Metabolites in Mice.

Food science of animal resources·2026
Same author

The first 24-h negative fluid balance is related to lower short-term mortality but higher AKI progression in critically ill heart failure: a retrospective cohort study.

Therapeutic advances in cardiovascular disease·2026

Related Experiment Video

Updated: Jun 24, 2025

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
09:11

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Published on: April 28, 2023

1.7K

hydroSIM: super-resolution speckle illumination microscopy with a hydrogel diffuser.

Zijun Gao1,2,3, Keyi Han1, Xuanwen Hua1

  • 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.

Biomedical Optics Express
|June 13, 2024
PubMed
Summary

A new super-resolution microscopy technique, hydrogel-based speckle-illumination microscopy (hydroSIM), simplifies cellular imaging. This cost-effective method achieves high-resolution 3D imaging on standard microscopes, making advanced cellular biology research more accessible.

More Related Videos

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
07:28

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Published on: November 4, 2021

2.8K
FRET Imaging in Three-dimensional Hydrogels
09:47

FRET Imaging in Three-dimensional Hydrogels

Published on: August 1, 2016

13.1K

Related Experiment Videos

Last Updated: Jun 24, 2025

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
09:11

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Published on: April 28, 2023

1.7K
Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
07:28

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Published on: November 4, 2021

2.8K
FRET Imaging in Three-dimensional Hydrogels
09:47

FRET Imaging in Three-dimensional Hydrogels

Published on: August 1, 2016

13.1K

Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Cellular Biology

Background:

  • Super-resolution microscopy provides critical insights into cellular structures and dynamics.
  • Structured illumination microscopy (SIM) offers a balance of resolution and speed but faces challenges in instrumentation complexity and alignment.
  • There is a need for simplified, accessible super-resolution techniques for broader adoption in biological research.

Purpose of the Study:

  • To introduce a simplified super-resolution microscopy method using hydrogel diffusers, termed hydroSIM.
  • To demonstrate the feasibility and performance of hydroSIM for 3D imaging of biological samples.
  • To present hydroSIM as a cost-effective and user-friendly alternative to existing super-resolution techniques.

Main Methods:

  • Development of hydroSIM utilizing the scattering and optical transmission properties of hydrogel materials.
  • Integration of the hydrogel diffuser into a standard epi-fluorescence microscopy platform.
  • Testing and validation of the hydroSIM system with various phantom and biological samples.

Main Results:

  • The hydroSIM system effectively achieved 3D resolution doubling, surpassing the diffraction limit.
  • Demonstrated capabilities include high-contrast imaging and optical sectioning, crucial for detailed cellular visualization.
  • Successful imaging of both phantom and diverse biological specimens confirmed the system's versatility.

Conclusions:

  • HydroSIM offers a simplified, plug-in solution for super-resolution imaging.
  • The technique is cost-effective, biocompatible, and user-accessible, lowering barriers to advanced microscopy.
  • HydroSIM is poised to significantly advance biomedical imaging and various cellular biology applications.