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Updated: Jun 24, 2025

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
Published on: February 28, 2018
Primer selection impacts the evaluation of microecological patterns in environmental microbiomes
Jintao He1, Tong Zhou2, Xiaoqiang Shen1
1Max Planck Partner Group, Institute of Sericulture and Apiculture, Faculty of Agriculture, Life and Environmental Sciences Zhejiang University Hangzhou China.
Abstract:
This study revealed that primer selection substantially influences the taxonomic and predicted functional composition and the characterization of microecological patterns, which was not alleviated by close-reference clustering. Biases were relatively consistent across different habitats in community profiling but not in microecological patterns. These primer biases could be attributed to multiple aspects, including taxa specificity, regional hypervariability, and amplification efficiency.
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