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Related Concept Videos

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  3. Research Domains
  5. Engineering
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  9. Air Pollution Modelling And Control
  11. Comprehensive Analysis Of Resilience Of Human Airway Epithelial Barrier Against Short-term Pm2.5 Inorganic Dust Exposure Using In Vitro Microfluidic Chip And Ex Vivo Human Airway Models.
  1. Home
  3. Research Domains
  5. Engineering
  7. Environmental Engineering
  9. Air Pollution Modelling And Control
  11. Comprehensive Analysis Of Resilience Of Human Airway Epithelial Barrier Against Short-term Pm2.5 Inorganic Dust Exposure Using In Vitro Microfluidic Chip And Ex Vivo Human Airway Models.

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Comprehensive analysis of resilience of human airway epithelial barrier against short-term PM2.5 inorganic dust exposure using in vitro microfluidic chip and ex vivo human airway models.

Ozlem Goksel1,2, Meryem Irem Sipahi3, Sena Yanasik4

  • 1Department of Pulmonary Medicine, Division of Immunology and Allergy, Laboratory of Occupational & Environmental Respiratory Diseases and Asthma, Faculty of Medicine, Ege University, Izmir, Turkey.

Allergy
|June 13, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
PM2.5airway epithelial barriermicrofluidic systemsparticulate matter

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Exposure to fine particulate matter (PM2.5) significantly damages airway epithelial barriers. Both an airway epithelial barrier (AEB)-on-a-chip and ex vivo tissue models showed reduced cell viability and increased inflammation after PM2.5 exposure.

Area of Science:

  • Environmental Health
  • Toxicology
  • Biomaterials

Background:

  • World Health Organization guidelines for fine particulate matter (PM2.5) are frequently exceeded globally.
  • Climate change exacerbates exposure through extreme weather and dust transport.
  • Inorganic silica PM2.5 poses a significant risk to respiratory health.

Purpose of the Study:

  • To evaluate the impact of respirable inorganic silica PM2.5 on epithelial barrier integrity.
  • To compare the effects in a biomimetic airway epithelial barrier (AEB)-on-a-chip model and ex vivo human airway tissue.
  • To assess the resilience of the airway epithelial barrier under simulated exposure conditions.

Main Methods:

  • Utilized an AEB-on-a-chip platform and ex vivo human bronchoscopy bronchial tissue slices.
silica particles
  • Exposed both models to silica particles (PM2.5) at 800 μg/mL for 72 hours.
  • Assessed cell viability, morphology, barrier integrity, permeability, and inflammation using various assays and CFD simulations.

    • Main Results:

    • PM2.5 exposure disrupted AEB integrity, increasing permeability and decreasing cell adhesion markers (ZO-1, Vinculin, ACE2, CD31).
    • Impaired cell viability and increased pro-inflammatory markers (IFNs, IL-6, TNF-α) were observed, particularly under dynamic conditions in the AEB-on-a-chip model.
    • Ex vivo tissues showed decreased viability, impaired integrity (reduced Vinculin, E-cadherin), and elevated inflammatory markers.

    Conclusions:

    • The study demonstrates the epithelial barrier's resilience to PM2.5 concentrations and durations relevant to extreme weather events.
    • Both the AEB-on-a-chip and ex vivo models effectively simulated PM2.5 exposure effects.
    • Lung-on-a-chip models show promise as reliable platforms for studying air pollution impacts on respiratory health.