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Related Concept Videos

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Updated: Jun 24, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
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Plasma/Serum Proteomics based on Mass Spectrometry.

Yiying Zhu1

  • 1Department of Chemistry, Tsinghua University, Beijing, China.

Protein and Peptide Letters
|June 13, 2024
PubMed
Summary

Discovering new blood biomarkers is crucial for precision medicine. This review focuses on mass spectrometry (MS) techniques for plasma/serum proteomics to advance biomarker discovery and diagnostic assay development.

Keywords:
Proteomicsbiomarkerclinical assay.diagnostic testliquid biopsymass spectrometryplasma/serum

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Area of Science:

  • Biochemistry
  • Proteomics
  • Clinical Diagnostics

Background:

  • Blood biomarkers are vital for disease diagnosis, prognosis, and treatment monitoring.
  • Precision medicine necessitates novel blood biomarkers and sensitive diagnostic assays.
  • Plasma/serum proteomics is gaining traction for biomarker discovery.

Purpose of the Study:

  • To review advancements in blood proteomics technologies, particularly mass spectrometry (MS).
  • To guide researchers in biomarker discovery and clinical diagnostic assay development.
  • To summarize protein analytes and sample preparation methods for MS analysis.

Main Methods:

  • Comprehensive review of mass spectrometry (MS) based blood proteomics technologies.
  • Analysis of existing blood biomarkers and MS-based diagnostic assays.
  • Summary of sample preparation techniques for total proteome, post-translational modifications, and extracellular vesicles.

Main Results:

  • Mass spectrometry (MS) offers powerful capabilities for blood biomarker discovery.
  • Screening multiple protein analytes simultaneously enhances biomarker discovery success rates.
  • Automation in sample preparation and MS techniques are key trends.

Conclusions:

  • Mass spectrometry (MS) is a pivotal technology for advancing blood biomarker discovery and diagnostics.
  • Integrated approaches analyzing multiple analytes and automated workflows will shape future blood tests.
  • Further development in MS techniques promises improved precision medicine applications.