Related Experiment Video
Updated: Jun 24, 2025

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Apollon: a deoxyribozyme that generates a yellow product.
Martin Volek1,2, Jaroslav Kurfürst1,3, Milan Kožíšek1
1Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague 166 10, Czech Republic.
Researchers developed Apollon, a novel deoxyribozyme for colorimetric assays. This self-phosphorylating enzyme converts a colorless substrate to a yellow product, enabling simple and cost-effective detection in various applications.
Area of Science:
- Biochemistry
- Molecular Biology
- Chemical Biology
Background:
- Colorimetric assays offer simple, inexpensive experimental monitoring.
- Enzyme-based detection systems are crucial for biochemical analysis.
Purpose of the Study:
- To identify and characterize a novel deoxyribozyme for colorimetric detection.
- To develop a self-phosphorylating kinase deoxyribozyme for signal generation.
Main Methods:
- In vitro selection and high-throughput sequencing to identify the deoxyribozyme.
- Biochemical assays and NMR spectroscopy for characterization.
- Engineering of deoxyribozyme variants for specific detection.
Main Results:
- A self-phosphorylating deoxyribozyme, named Apollon, was identified.
- Apollon converts pNPP to pNP, producing a colorimetric signal.
- Engineered Apollon detected specific oligonucleotides and nucleases in homogenous assays.
Conclusions:
- Apollon provides a versatile tool for colorimetric detection.
- The deoxyribozyme enables cost-effective, high-throughput screening.
- Apollon is suitable for applications lacking specialized equipment.
More Related Videos
11:37Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
10:59Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
Related Concept Videos
ATP and Macromolecule Synthesis
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
Ribozymes
Ribozymes can...
Glycolysis: Pay-off Phase
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...