Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus

A portion of the genome of the nuclear polyhedrosis virus of Bombyx mori has been cloned. This part of the viral genome contains the gene encoding the viral occlusion body protein, polyhedrin. The polyhedrin gene has been sequenced in its entirety together with some of its 5' and 3' flanking sequences. The primary structure of polyhedrin predicted from the nucleotide sequence of the gene was found to be somewhat different from the one reported previously for the authentic protein (E. A. Kozlov, T. L. Levitina, N. M. Gusak, and S. B. Serebryani, Bioorg. Khim., 7:1008-1015, 1981; S. B. Serebryani, T. L. Levitina, M. L. Kautsman, Y. L. Radavski, N. M. Gusak, M. N. Ovander, N. V. Sucharenko, and E. A. Kozlov, J. Invertebr. Pathol., 30:442-443, 1977). Comparison of the primary structures of the polyhedrin of the nuclear polyhedrosis virus of B. mori with that of Autographa californica suggests that considerable selective pressure has been exercised at the protein level during evolution. Nucleotide sequence comparisons of the two structural genes reveal that the coding sequences have diverged significantly through the accumulation of silent and replacement substitutions. In contrast, a remarkable degree of sequence conservation was found to exist in the domains corresponding to the 5' and 3' noncoding regions of the polyhedrin mRNAs.