Is minimally-invasive sampling the future of persistent organic pollutant (POP) research in birds? A meta-analysis on...

Persistent organic pollutants (POPs) bioaccumulate in the food chain and can cause ecotoxicity. In wild bird populations, various tissues are used to determine POP levels, including invasive (e.g., brain, fat, kidney, liver, muscle) and minimally-invasive tissues (e.g., blood, feather, preen oil). Minimally-invasive sampling, which does not require the death of the animal, opens new prospects for sampling birds as sentinels of environmental pollution and its consequences on fitness. However, POP variability between tissues is understudied, which is an essential prerequisite for making a reasoned choice about which tissues to sample. Here, we performed a meta-analysis of eight tissues across 115 studies comparing tissues across POP groups. We demonstrate increased use of minimally-invasive measures between 1974 and 2020. When grouping tissue correlations into three groups, "invasive:invasive", "invasive:minimally-invasive" and "minimally-invasive:minimally-invasive", we found that all three groups produced moderate to strong positive correlations with no difference seen between comparison groups. We demonstrate (1) lower POP concentrations in preen oil than fat, but no difference in detection frequencies, supporting preen oil use; (2) blood showed high concentration variability dependent on POP group but detection frequencies were comparable to liver and kidney; and (3) feathers demonstrated a significantly lower detection frequency than other matrices measured. By further researching minimally-invasive tissues, we increase our understanding of whether minimally-invasive tissues are ecologically representative of body-level toxicity. Our study supports blood and preen oil as substitutes for invasive measures when sampling living bird populations as they represent internal POP concentrations and provide significant benefits both practically and ethically.