Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K
Hematopoiesis01:21

Hematopoiesis

5.2K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.2K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.2K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.2K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

3.9K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A practically efficient algorithm for identifying critical control proteins in directed probabilistic biological networks.

NPJ systems biology and applications·2024
Same author

Target control of linear directed networks based on the path cover problem.

Scientific reports·2024
Same author

Measuring criticality in control of complex biological networks.

NPJ systems biology and applications·2024
Same author

Probabilistic Critical Controllability Analysis of Protein Interaction Networks Integrating Normal Brain Ageing Gene Expression Profiles.

International journal of molecular sciences·2021
Same author

Uncovering and classifying the role of driven nodes in control of complex networks.

Scientific reports·2021
Same author

An Overview of Bioinformatics Methods for Analyzing Autism Spectrum Disorders.

Current pharmaceutical design·2019

Related Experiment Video

Updated: Jun 24, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

Determining cellular lineage directed networks in hematopoiesis using single-cell transcriptomic data and

Tomoshiro Ochiai1, Jose C Nacher2

  • 1Faculty of Social Information Studies, Otsuma Women's University, 12 Sanban-cho, Chiyoda-ku, Tokyo 102-8357, Japan.

Bio Systems
|June 13, 2024
PubMed
Summary

Single-cell RNA sequencing reveals a dual origin for basophil cells in hematopoiesis. This new directional network model enhances our understanding of blood cell development and provides a framework for future biomedical research.

Keywords:
Complex networksDirected network predictionHematopoiesisSingle-cell transcriptomics dataVolatility-constrained correlation

More Related Videos

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

1.5K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K

Related Experiment Videos

Last Updated: Jun 24, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

1.5K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K

Area of Science:

  • Genomics and Bioinformatics
  • Developmental Biology
  • Hematology

Background:

  • Single-cell RNA sequencing (scRNA-seq) advances cellular process understanding by analyzing individual cell expression profiles.
  • scRNA-seq aids in discovering cell types, gene functions, differentiation, and trajectory inference in biological processes like hematopoiesis.
  • Previous scRNA-seq analyses of mouse bone marrow established hematopoietic lineage network models, but predicted undirected cell trajectories.

Purpose of the Study:

  • To apply the Volatility Constrained (VC) correlation method for predicting directed cell trajectories in hematopoiesis.
  • To resolve the debate on basophil cell origins by inferring causal relationships between hematopoietic cell types.
  • To develop a comprehensive and directional network map of the hematopoietic process.

Main Methods:

  • Utilized the Volatility Constrained (VC) correlation method for network analysis.
  • Applied scRNA-seq data from mouse bone marrow cells.
  • Inferred directional causality between cell types within the hematopoietic lineage.

Main Results:

  • Predicted a directed network map of hematopoietic cell trajectories, overcoming limitations of previous undirected models.
  • Identified a dual origin for basophil cells, originating from both granulocyte/macrophage and erythrocyte progenitors.
  • Highlighted an under-explored trajectory from erythrocyte progenitors contributing to basophil development.

Conclusions:

  • The VC correlation method provides a robust framework for inferring directional causality in cellular processes.
  • Findings suggest a dual origin of basophils, offering new insights into hematopoietic development.
  • The developed directional network framework can significantly advance hematopoiesis research and biomedical applications.