Towards minimizing second-generation mis-identification of Blastocystis
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Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
Published on: February 28, 2019
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Christen Rune Stensvold1, Lee O'Brien Andersen1, Eleni Gentekaki2
1Laboratory of Parasitology, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
Trends in Parasitology
|June 13, 2024
Abstract:
At least 1-2% of DNA sequences annotated as Blastocystis in GenBank represent organisms other than Blastocystis or sequence artefacts. As well as being biologically incorrect, such practice can lead to overestimates of genetic diversity, underestimated host specificity, and incorrect classification of samples tested for Blastocystis using DNA-based methods.
