Related Experiment Video
Updated: Jun 24, 2025
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
12.2K
Optimal design of synthetic circular RNAs
Seo-Won Choi1, Jin-Wu Nam2,3,4,5
1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
Experimental & Molecular Medicine
|June 13, 2024
Summary
This review details optimizing circular RNA (ribonucleic acid) design, synthesis, and delivery. It covers strategies and applications for producing these versatile molecules efficiently.
Area of Science:
- Molecular Biology
- RNA Therapeutics
Background:
- Circular RNAs (circRNAs) are unique single-stranded RNAs formed by back-splicing.
- Their versatility drives increased demand for in vivo and in vitro expression.
- Current literature lacks a comprehensive review on optimizing circRNA production.
Purpose of the Study:
- To provide a comprehensive review on the optimization of circular RNA design, synthesis, and delivery.
- To summarize available strategies for exogenous circular RNA production.
- To highlight potential applications of circular RNAs.
Main Methods:
- Literature review focusing on circular RNA synthesis and design.
- Analysis of various circularization strategies.
- Exploration of delivery methods for circular RNAs.
Main Results:
- Identification of key factors for optimizing circRNA design and synthesis.
- Summary of diverse circularization techniques and their efficiencies.
- Overview of potential therapeutic and research applications.
Conclusions:
- Optimized design, synthesis, and delivery are crucial for effective circular RNA utilization.
- A range of strategies exist for producing high-quality circular RNAs.
- Circular RNAs hold significant promise for various biological and therapeutic applications.
Related Concept Videos
02:30siRNA - Small Interfering RNAs
16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
03:21RNA-seq
9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K