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Updated: Jun 24, 2025

Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
Optimal design of synthetic circular RNAs
Seo-Won Choi1, Jin-Wu Nam2,3,4,5
1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
Abstract:
Circular RNAs are an unusual class of single-stranded RNAs whose ends are covalently linked via back-splicing. Due to their versatility, the need to express circular RNAs in vivo and in vitro has increased. Efforts have been made to efficiently and precisely synthesize circular RNAs. However, a review on the optimization of the processes of circular RNA design, synthesis, and delivery is lacking. Our review highlights the multifaceted aspects considered when producing optimal circular RNAs and summarizes the available options for each step of exogenous circular RNA design and synthesis, including circularization strategies. Additionally, this review describes several potential applications of circular RNAs.
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