Building RNA-Mediated Artificial Signaling Pathways between Endogenous Genes.

Area of Science:

• Synthetic biology
• Genetic engineering
• Molecular biology

Background:

• Cellular responses are governed by complex genetic networks and signaling pathways.
• Artificial signaling pathways (ASPs) offer programmable control over cellular functions.
• Connecting endogenous genes via ASPs is crucial for modifying genetic networks and addressing aberrant gene expression.

Purpose of the Study:

• To review the construction of RNA-mediated ASPs between endogenous genes for responding to differentiated RNA expression.
• To highlight the development of efficient strategies for manipulating cell responses using RNA-mediated ASPs in mammalian cells.
• To discuss the potential applications of these engineered signaling circuits.

Main Methods:

• Utilizing RNA-based genetic circuits, including RNA toehold switches, siRNA, and CRISPR systems.
• Developing strategies for manipulating endogenous RNAs to establish artificial signaling pathways.
• Engineering customized cell signaling circuits from bacterial to mammalian cells.

Main Results:

• Demonstrated the construction of RNA-mediated ASPs between endogenous genes.
• Showcased the programmability and tunability of synthetic RNA circuits for diverse functionalities.
• Advanced the manipulation of naturally occurring RNAs for endogenous gene connections, particularly in mammalian systems.

Conclusions:

• RNA-mediated ASPs provide a powerful platform for engineering cellular behaviors and developing novel therapeutic strategies.
• The developed strategies enable precise control over gene expression and cellular responses.
• Further research holds promise for fundamental and translational applications in medicine and biotechnology.