Presence of microtubule-associated protein 2 in chromaffin cells

We have examined bovine adrenal chromaffin cells in culture for the presence of the microtubule-associated protein 2. Chromaffin cells could be identified in culture on the basis of their staining with antibodies directed against secretory granule proteins. Using immunoperoxidase staining microtubule-associated protein 2 immunoreactivity was demonstrated to be present in chromaffin cells but not fibroblasts in culture. Microtubule-associated protein 2 immunoreactivity was present in the cell body, processes and varicosities of the chromaffin cells. Microtubule-associated protein 2 polypeptides were shown to be present in an adrenal medullary homogenate but not chromaffin granule membranes by immunoblotting. The results indicate that the neuronal cytoskeletal polypeptide microtubule-associated protein 2 is present in adrenal chromaffin cells. The presence of microtubule-associated protein 2 in both neurons and chromaffin cells may be related to their common embryonic origin.