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Related Experiment Videos

Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope.

N B Datyner, G A Gintant, I S Cohen

    Pflugers Archiv : European Journal of Physiology
    |March 1, 1985
    PubMed
    Summary

    This study presents a compact, affordable temperature-controlled bath for electrophysiology. The design ensures precise temperature uniformity for isolated cell studies, minimizing electrical interference for clearer results.

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    Area of Science:

    • Biophysics
    • Neuroscience
    • Cell Biology

    Background:

    • Electrophysiological studies require precise control of the cellular microenvironment.
    • Existing temperature control systems can be expensive or introduce electrical noise.
    • Small-volume baths are crucial for studying limited biological samples.

    Purpose of the Study:

    • To describe the design of a novel, low-cost, small-volume temperature-controlled bath.
    • To achieve stable and uniform temperature (+/- 1°C) for electrophysiological recordings.
    • To facilitate microelectrode access and unobstructed microscopic visualization of isolated cells.

    Main Methods:

    • A 0.25 ml bath chamber with a 0.6 X 1 cm area was designed.
    • A finely stranded heater was integrated beneath the bath chamber, between glass coverslips.

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  • A battery-operated feedback temperature controller and solution pre-heater were employed.

    • Main Results:

    • Uniform temperature control was achieved from ambient to ~37°C.
    • Temperature stability was maintained at +/- 1°C across varying flow rates (0-2 ml/min).
    • The design allows for easy microelectrode access and clear microscopic viewing.

    Conclusions:

    • The described temperature-controlled bath is a cost-effective and efficient solution for electrophysiological studies.
    • Its design minimizes electrical interference, enhancing signal quality.
    • This system is suitable for research involving isolated cells and microelectrode techniques.