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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Improved breast milk proteome coverage by DIA based LC-MS/MS method.

Jenni Viitaharju1, Lauri Polari2,3, Otto Kauko4

  • 1Institute of Biomedicine, Research Centre for Integrative Physiology, and Pharmacology, Faculty of Medicine, University of Turku, Turku, Finland.

Proteomics
|June 14, 2024
PubMed
Summary
This summary is machine-generated.

This study enhanced breast milk proteome analysis, identifying over 2400 proteins using a hybrid method. Researchers found significant protein differences in obese and overweight mothers, linking body mass index (BMI) to breast milk composition.

Keywords:
breast milkcolostrummass spectrometryobesityproteomics

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Area of Science:

  • Proteomics
  • Human Milk Biology
  • Biochemistry

Background:

  • Breast milk contains vital bioactive factors, including proteins, but proteomic analysis is challenging due to concentration ranges and interfering substances.
  • Previous studies identified fewer than 1000 proteins, limiting comprehensive understanding of the breast milk proteome.
  • Advanced separation technologies and optimized procedures are crucial for deeper insights into milk proteome composition.

Purpose of the Study:

  • To improve breast milk proteome analysis by employing and comparing three distinct protein quantification methods.
  • To identify proteins in breast milk plasma that are associated with maternal body mass index (BMI).
  • To evaluate the efficacy of high field asymmetric waveform ion mobility spectrometry (FAIMS) in enhancing protein identification.

Main Methods:

  • Utilized three protein quantification strategies: direct data-independent acquisition (DIA), library-based DIA, and a hybrid DIA approach.
  • Analyzed breast milk samples from 53 mothers using optimized proteomic procedures.
  • Applied high field asymmetric waveform ion mobility spectrometry (FAIMS) coupled with mass spectrometry on a subset of samples.

Main Results:

  • The hybrid DIA method identified an average of 2400 proteins, significantly exceeding previous study limitations.
  • Identified 210 significantly different proteins between obese/overweight mothers and normal weight mothers, indicating BMI-associated variations.
  • FAIMS coupled with Orbitrap Fusion Lumos mass spectrometry increased protein identifications by 41.7% compared to Q Exactive HF.

Conclusions:

  • The hybrid DIA method offers a substantial advancement in breast milk proteome profiling, enabling deeper analysis.
  • Maternal BMI is associated with significant alterations in the breast milk proteome.
  • FAIMS represents a promising technology for enhancing proteomic depth in complex biological samples like breast milk.